众所周知，在细胞膜或者膜结构上有很多跨膜蛋白，它们有些片段在细胞外，有些片段在细胞内，分别发挥着不同的功能。对于研究比较程度的蛋白指标而言，小伙伴们都可以通过成熟的数据库平台进行查阅，比如：我们的老朋友平台：Uniprot （https://www.uniprot.org/）
下面均以EGFR（P00533）蛋白为例，展示如何查找或者预测蛋白质的胞内与胞外分布：
已知蛋白
①查找蛋白指标：VEGF，找到对应的种属蛋白：
②点击亚细胞定位（Subcellular location）
③ 往下翻找到Topology（拓扑学），了解胞内，胞外分布：
未知蛋白
①打开Topocons网站：
http://topcons.cbr.su.se/
② 如上图，在文本框中导入相关序列的FASTA格式序列（必须含>sp…相关内容），建议提供您的邮箱（结果预测出来后，会给您发邮件，如下图），然后点击提交（Submit）即可。
③ 观察结果：蓝色表示胞外部分（26-644 aa）；红色表示胞内部分（666-1210 aa）。
通过上述2种方法对比发现，其实Topcons预测结果，与实验验证的数据库Uniprot结果非常接近，可信度非常棒！
以上希望可以帮助到更多的科研小伙伴们，更多资讯，欢迎持续关注每日生物评论（Bio-review）哦~
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询问下蛋白质的细胞内/细胞外应该叫蛋白质的什么属性呢？是否有英文方面的专业术语表示？