上周一的文章中，我们聊到了两种不同的大肠杆菌转化的方法，化学转化和电穿孔转化（详情点这里 ）。在我们知道细胞转化的方法之后，就应该考虑选择哪种感受态细胞进行转化。市面上的感受态细胞种类相当多，到底选哪一个是由你要进行的实验来决定的，不同基因型的大肠菌株自然是可以满足不同的实验目的。小编在本文简单总结常用的那几种基因型（Genotype）的大肠杆菌细胞，以及他们能满足的实验目的。至于在具体实验中应该选择哪一种基因型的细胞，小编觉得最好的方法是和导师/师兄/师姐讨论，问问他们曾经用的是什么细胞以及用的效果怎么样，再结合自己的实验目的选出最合适的细胞种类。
实验目的Ⅰ: 保证外源质粒的完整性
实验目的Ⅱ: 提高转化效率
实验目的Ⅲ: 满足不同的蛋白表达机制
以上只是众多改造细胞中的一小部分，还有很多不常见的变异菌株，可适用于更加特殊的实验目的，在这里小编就不多说了。还是那句话，在你的实验中具体使用哪种菌株，和用过的有经验的人讨论讨论，一般来说就能找到自己能用的细胞种类。
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