相信大家对单克隆抗体 (monoclonal antibodies,mAbs)一定不陌生,而且不少实验需要用到它们。这些抗体通常是通过杂交瘤细胞系统生产出来的。这个方法最早是由 Köhler 和Milstein 在 1975年发明的1。简单来说,就是一只实验小鼠为所需要产生的抗体而进行免疫。将小鼠的脾细胞(spleen cells)-主要由免疫系统中B细胞组成,和特殊的骨髓瘤细胞(myeloma cells)融合,就会产生持续增殖细胞(immortal hybrid cell)。这些细胞被称作杂交瘤细胞(hybridoma),它们可以产生特定的单克隆抗体。
虽然这个技术被广泛使用,但是它同样有许多弊端。为了克服这些问题,重组抗体(recombinant antibodies,rAbs) 应运而生,并且颠覆了整个单克隆抗体的生产模式。那么今儿小编就给大家简单介绍一下rAbs的好处!首先,什么是重组抗体呢?rAbs 是在活生物体之外(in vitro)的实验室中通过基因重组的技术构建的。它们是不受免疫系统的限制的,而且这些重组抗体可以在任何使用mAbs的应用中使用。
那么,重组抗体的好处是什么呢?
1. 它们需要更少的提纯抗原就能被生产出来;
2. 生产的时间从以月份来计量变成以周计量,而且是高通量的(high-throughput);
3. 不像mAbs,rAbs 可以根据任何一种抗原被构建出来,包括一些小鼠免疫系统不能识别的抗原;或是不能激发免疫系统的抗原;和一些对动物体有毒的分子;
4. rAbs 可以防止由小鼠单克隆抗体激发的人类免疫系统反应。mABs,即使是已经“人类化”(humanized)的(图1),也会被人类免疫系统识别,会造成超敏反应(hypersensitivity reaction)。这个反应是免疫反应产生作用分子移除外来抗原的过程。在特殊的状况下,发炎反应可能导致明显的组织伤害甚至死亡。
5. rABs 可以有几种形式:Fab片段,单链变异区片段抗体(single-chain variable region fragments,scFv),双价小抗体(diabodies,dimeric scFvs)等等 (图2)。不但形式多种多样,而且表达的系统也很广泛,例如大肠杆菌,哺乳动物细胞,酵母,昆虫细胞,甚至是植物细胞。
References
1. Köhler G., Milstein C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495-97.
2. Patricia A., Herrington-Symes1, Monika Farys1, Hanieh Khalili (2013). Steve Brocchini Antibody fragments: Prolonging circulation half-life special issue-antibody research. Advances in Bioscience and Biotechnology.
图片来自网络,内容由BioEngX原创,如需转载,请联系管理员。
管理员邮箱:info@bioengx.org;管理员微信:bioengxadmin
гѓ—гѓ¬гѓ‰гѓ‹гѓігЃ®иіје…Ґ – г‚ўг‚ёг‚№гѓгѓћг‚¤г‚·гѓійЂљиІ©гЃ§иІ·гЃ€гЃѕгЃ™гЃ‹ г‚ёг‚№гѓгѓћгѓѓг‚Ї е‰ЇдЅњз”Ё
バイアグラの購入 – г‚·г‚ўгѓЄг‚№ гЃЉгЃ™гЃ™г‚Ѓ г‚·г‚ўгѓЄг‚№ еЂ‹дєєијёе…Ґ гЃЉгЃ™гЃ™г‚Ѓ
order crotamiton online – order generic eurax order aczone online
losartan price – order cephalexin 500mg pills order keflex 125mg generic
oral cleocin 150mg – buy indocin 50mg pill buy indomethacin 75mg pill
betnovate 20 gm drug – buy adapalene no prescription generic benoquin
deltasone 40mg us – buy prednisone 40mg online cheap zovirax cream
accutane where to buy – avlosulfon 100mg uk deltasone online buy
purchase omnicef pills – how to get omnicef without a prescription clindamycin generic
buy artane – artane for sale online order voltaren gel online
periactin oral – buy zanaflex without prescription tizanidine pills
buy mobic for sale – meloxicam order toradol 10mg for sale
ozobax canada – order baclofen 10mg sale order feldene generic
order mestinon sale – buy generic azathioprine 25mg azathioprine generic
rumalaya online buy – buy rumalaya without a prescription order generic elavil 10mg
cambia pills – brand aspirin 75 mg buy aspirin 75 mg online cheap
how to buy besivance – purchase carbocysteine order sildamax
order generic calcort – cheap calcort pills order generic brimonidine
imusporin price – order colchicine without prescription gloperba sale
They all appear to help equally generic cialis vs cialis
purchase duphalac bottles – brahmi order online buy betahistine 16mg
oxcarbazepine for sale – purchase pirfenidone online cheap purchase synthroid online cheap
cheap finasteride – buy finax sale alfuzosin tablet
buy cheap gasex – cheap gasex pills order diabecon generic
atorvastatin online buy – atorlip where to buy cost bystolic
buy cheap calan – purchase diltiazem sale buy tenoretic online
atenolol 100mg pills – order generic plavix buy coreg pill
order minoxidil online – cheap finpecia pills buy propecia sale
purchase leflunomide generic – alfacip usa buy cartidin for sale
purchase durex gel – buy durex gel online cheap xalatan
buy ascorbic acid without prescription – buy ferrous sulfate 100 mg sale cost compro
buy ondansetron without prescription – order ropinirole 1mg pill buy requip 2mg pill
buy generic flexeril 15mg – aricept without prescription order enalapril for sale
cytoxan canada – dimenhydrinate 50mg us order vastarel for sale
buy depakote 250mg pills – acetazolamide online topamax cheap
disopyramide phosphate medication – order chlorpromazine 50 mg sale thorazine pills
order hydroxyurea for sale – order disulfiram 500mg pills purchase robaxin generic
order nootropil 800mg generic – order secnidazole pill sinemet 10mg for sale
monograph online order – order cilostazol 100mg generic order cilostazol 100mg
feldene 20mg drug – exelon 6mg pills rivastigmine pills
buy dimenhydrinate 50mg – buy generic dimenhydrinate online buy risedronate 35mg
vasotec 5mg sale – vasotec 5mg tablet purchase latanoprost
dapagliflozin 10 mg pills – sinequan for sale buy acarbose generic
hydroquinone for sale online – buy hydroquinone no prescription dydrogesterone 10mg pills
cotrimoxazole generic – tobra 5mg drops tobra over the counter
purchase rabeprazole pills – buy domperidone pills for sale buy domperidone 10mg sale
biaxin somewhere – albenza marble cytotec pills alter
fludrocortisone pills myself – fludrocortisone brother lansoprazole pills ankh
ascorbic acid depress – ascorbic acid whole ascorbic acid history
promethazine ponder – promethazine yourself promethazine gratitude
priligy counter – dapoxetine statement priligy appear
claritin metal – loratadine physician claritin pills deed
loratadine medication great – loratadine medication afford claritin pills obtain
valacyclovir club – valacyclovir pills bathroom valtrex pills pleasure
prostatitis pills exercise – prostatitis medications perhaps prostatitis treatment english
treatment for uti beg – uti antibiotics generation uti treatment capture
asthma treatment occur – inhalers for asthma plenty asthma medication always
acne treatment ponder – acne treatment prove acne medication unseen
cialis soft tabs pills recall – viagra super active online shave viagra oral jelly brave
brand cialis sheet – tadora library penisole cheerful
cenforce barrel – tadacip pills hair brand viagra burn
dapoxetine foe – dapoxetine plan cialis with dapoxetine freeze
viagra professional online continue – levitra oral jelly online rate levitra oral jelly operation
zocor then – lipitor 80mg oral atorvastatin forever
rosuvastatin weep – pravachol buy roll caduet online want
purchase nitroglycerin generic – combipres drop order diovan generic
metoprolol online buy – buy inderal 20mg without prescription purchase nifedipine generic
hydrochlorothiazide without prescription – microzide ca order zebeta
famvir brand – buy acyclovir without a prescription buy valaciclovir 500mg for sale
lanoxin 250 mg generic – order labetalol pills buy furosemide 100mg generic
order ketoconazole 200 mg online cheap – buy mentax cheap buy sporanox pills for sale
purchase semaglutide without prescription – rybelsus online order desmopressin cost
order terbinafine 250mg generic – purchase forcan online buy griseofulvin without prescription
prandin pill – empagliflozin 25mg cost empagliflozin 25mg cheap
order micronase 2.5mg online cheap – glucotrol generic buy forxiga 10mg
purchase clarinex online – ketotifen pill buy ventolin 2mg sale
ivermectin 3mg – buy generic levofloxacin cefaclor uk
buy generic ventolin 4mg – theophylline pills theo-24 Cr price
buy zithromax sale – order tinidazole 300mg online how to get ciplox without a prescription
purchase cleocin pills – cleocin 150mg pills purchase chloramphenicol pills
amoxiclav pills – order myambutol 1000mg generic buy ciprofloxacin 500mg
how to get anafranil without a prescription – order remeron 15mg for sale order doxepin 25mg generic
hydroxyzine online – brand amitriptyline 10mg buy endep
order quetiapine generic – buy quetiapine 100mg pill cheap eskalith online
buy generic clozaril 50mg – buy generic frumil buy famotidine 20mg online
where can i buy cheap retrovir – biaxsig over the counter zyloprim pills
furosemide 40mg cheap – cheap capoten 25 mg generic capoten 25 mg
buy cheap generic ampicillin ampicillin usa buy amoxil medication
where to buy ivermectin for humans – order tetracycline without prescription tetracycline over the counter
valacyclovir generic – cheap nemasole sale buy acyclovir 800mg pills
purchase ciprofloxacin without prescription – ciprofloxacin 500 mg canada erythromycin over the counter
metronidazole cheap – where can i buy azithromycin purchase azithromycin pill
order cipro 1000mg pills – cephalexin tablet brand augmentin 625mg
order generic cipro 1000mg – buy generic cephalexin for sale cheap clavulanate
where to buy lipitor without a prescription order atorvastatin sale order lipitor 20mg pills