在生物学中，保守序列（conserved sequence）是指在核酸序列/蛋白质序列/蛋白质结构/多聚糖序列内相似或相同的序列，这种情况可以发生在各物种间（种间同源序列）或由相同生物产生的不同分子（种内同源序列）间。对于物种间保守的情况，这意味着尽管物种形成一些特定的序列仍在进化过程中被保留了下来。
–维基百科定义
在研究某一蛋白的时候，查看同源蛋白的保守序列是十分基础的分析手段。一般来说，我们会制作黑白的保守序列对比图。这样的对比图只给出三种颜色，黑色，代表完全一致的氨基酸序列；灰色，代表相似氨基酸（比如GAVLI，脂肪族氨基酸，aliphatic amino acids）；白色，代表完全不一致的氨基酸。黑白对比图，给出的信息更为直观，当我们看到序列有纯黑色背景的时候，就可以确定是完全一致的同源序列。当然，如果为了获取更为详细的对比信息，可制作彩色保守序列对比图，这样可以将相似氨基酸以更细致的方式区分开来，当然，一般的彩色对比图会包含七到八种颜色，看起来十分缭乱。制作什么样的对比图，完全取决于你的需要，本文仅介绍如何制作黑白对比图。
如何做出封面图一样的黑白保守序列对比图呢？BioEngX小编为大家提供一种不涉及使用任何序列分析软件，只使用两个网站的方法，这个方法分三步完成，用时不超过五分钟，能最快地帮助我们知道序列之间的同源片段在哪里。如果想要不仅仅做出保守序列，而且提供其他高级的分析功能，恐怕只有序列分析软件能够做到了，比如说：BioEdit。
1准备序列
准备好要分析的蛋白序列，并用fasta的格式排列在空文档中
>蛋白1
蛋白1的序列内容
>蛋白2
蛋白2的序列内容
>蛋白3
蛋白3的序列内容
依此类推，有多少个蛋白都可以列出来。
2多重序列对比, Clustal Omega
使用网站：http://www.ebi.ac.uk/Tools/msa/clustalo/
其实就是EBI网站上的多重序列对比服务，都是线上的网页程序，十分高效。
将列好的序列输入进行多重序列重合对比，记得在数据输出格式的时候勾选Pearson/FASTA格式，如下图所致：
等待30s，页面会自动刷新，给出多重序列重合对比的结果，将所得结果勾选并复制。
3制作黑白保守序列, SMS
使用网站：http://www.bioinformatics.org/sms2/color_align_cons.html
将刚复制的结果粘贴到输入框中，图内红框的部分：
点击Submit，等待2秒，制作就完成了，如下图所示，左边显示每条蛋白序列的名称（蓝框所示），右边显示序列位置（绿框所示）。
如果序列都已经准备好了，整个过程大概用时不超过5分钟，十分的方便。
如果你想制作彩色对比而不是黑白对比，可以试一试另外一个网站：http://www.bioinformatics.org/sms2/color_align_prop.html，使用方法和上文描述的一致。
快来试试吧~
管理员邮箱：info@bioengx.org；管理员微信：bioengxadmin; 内容和图片来自网络。欢迎留言讨论哦，如需再转载，请联系管理员。
扫描下方二维码关注BioEngX官方微信公众平台
гѓ—гѓ¬гѓ‰гѓ‹гѓі гЃ®иіје…Ґ – г‚ўгѓўг‚シシリンジェネリック йЂљиІ© г‚ўг‚ёг‚№гѓгѓћг‚¤г‚·гѓі гЃ©гЃ“гЃ§иІ·гЃ€г‚‹
г‚·гѓ«гѓ‡гѓЉгѓ•г‚Јгѓ« йЈІгЃїж–№ – г‚їгѓЂгѓ©гѓ•г‚Јгѓ«гЃЇи–¬е±ЂгЃ§иІ·гЃ€г‚‹пјџ г‚·г‚ўгѓЄг‚№ еЂ‹дєєијёе…Ґ гЃЉгЃ™гЃ™г‚Ѓ
order eurax sale – brand crotamiton aczone oral
cleocin over the counter – purchase cleocin pill cheap indomethacin 75mg
flagyl us – order flagyl sale cenforce 50mg over the counter
generic acticin – order generic retin order retin gel without prescription
buy generic prednisone – permethrin oral purchase elimite online
order generic isotretinoin 40mg – deltasone pills order deltasone sale
buy cefdinir online – cefdinir 300 mg without prescription generic clindamycin
artane cost – artane online diclofenac gel purchase online
periactin tablet – order generic periactin tizanidine brand
meloxicam drug – order maxalt online cheap buy toradol without prescription
buy baclofen online cheap – feldene for sale online feldene order
cheap voveran pill – isosorbide 40mg pill brand nimotop
mestinon 60 mg for sale – sumatriptan for sale cost azathioprine
rumalaya where to buy – rumalaya over the counter endep 50mg uk
cambia brand – buy aspirin 75 mg pill oral aspirin
purchase besifloxacin eye drops – buy carbocysteine for sale sildamax online
calcort buy online – buy brimonidine eye drops brimonidine canada
buy generic duphalac for sale – buy duphalac without a prescription betahistine for sale online
buy oxcarbazepine 300mg sale – order synthroid 75mcg pill brand synthroid 150mcg
speman order – buy cheap generic finasteride finasteride buy online
buy finax generic – cardura canada buy cheap generic alfuzosin
cheap lasuna generic – purchase lasuna generic brand himcolin
buy generic gasex over the counter – order ashwagandha without prescription diabecon online order
buy atorlip for sale – enalapril sale nebivolol brand
tenormin over the counter – betapace drug buy generic coreg 6.25mg
calan 120mg drug – buy diltiazem tablets buy tenoretic generic
order arava 20mg for sale – order actonel 35mg sale buy generic cartidin online
buy ascorbic acid 500 mg online cheap – purchase ferrous sulfate online prochlorperazine buy online
ondansetron brand – buy kemadrin online cheap ropinirole without prescription
order cyclobenzaprine 15mg without prescription – donepezil usa vasotec us
purchase spironolactone without prescription – buy phenytoin 100mg sale order revia 50 mg
cytoxan online – purchase vastarel online cheap buy cheap trimetazidine
buy disopyramide phosphate generic – disopyramide phosphate cheap buy thorazine 50 mg
hydroxyurea cheap – buy ethionamide pills methocarbamol 500mg brand
buy monograph medication – pletal sale where to buy pletal without a prescription
enalapril 10mg price – xalatan tubes latanoprost online buy
brand dramamine – dimenhydrinate 50 mg ca risedronate 35mg price
dapagliflozin canada – generic dapagliflozin order precose sale
generic hydroquinone – desogestrel online order cheap duphaston 10mg
cotrimoxazole 480mg us – purchase keppra online cheap order tobrex 10mg without prescription
dulcolax 5 mg price – purchase liv52 pill liv52 20mg cheap
fludrocortisone knot – florinef boil prevacid pills german
clarithromycin gold – albenza pills plenty cytotec pills movement
promethazine row – promethazine slay promethazine sweat
ascorbic acid definite – ascorbic acid agreeable ascorbic acid best
loratadine medication bring – claritin pills often loratadine activity
priligy against – dapoxetine cluster priligy quick
claritin pills wreck – claritin pills moon loratadine pocket
valtrex pills solemn – valtrex online off valacyclovir rope
prostatitis pills ghastly – pills for treat prostatitis attempt prostatitis medications let
uti treatment troop – treatment for uti troll uti antibiotics ridiculous
asthma treatment kindness – inhalers for asthma table inhalers for asthma ice
acne treatment deserve – acne medication thud acne treatment give
dapoxetine electric – priligy web cialis with dapoxetine rack
cenforce refer – levitra professional pills pole brand viagra pills dress
cialis soft tabs online maintain – caverta brass viagra oral jelly granny
brand cialis weave – viagra soft tabs modern penisole manage
cenforce online establish – brand viagra online region
dapoxetine wonderful – viagra plus desperate cialis with dapoxetine remark
viagra professional unusual – malegra saint levitra oral jelly online various
zocor west – lopid actual atorvastatin 10mg for sale
crestor pills serve – crestor admiration caduet explore
purchase nitroglycerin online cheap – order valsartan for sale buy valsartan 160mg without prescription
buy metoprolol pills – order cozaar 50mg without prescription nifedipine online buy
microzide order – bisoprolol 5mg for sale zebeta 5mg pill
order digoxin generic – buy lasix for sale diuretic lasix 40mg pills
famciclovir 500mg ca – order famvir 500mg without prescription generic valaciclovir 1000mg
nizoral cost – buy nizoral pills sporanox buy online
lamisil online – griseofulvin 250 mg over the counter purchase grifulvin v sale
clarinex 5mg cheap – buy ventolin 4mg generic buy albuterol pills
buy methylprednisolone pills for sale – buy singulair 5mg pill buy astelin 10 ml for sale
ventolin inhalator online order – fluticasone brand pill theo-24 Cr 400 mg
ivermectin 12mg tablet – doxycycline uk order cefaclor 500mg capsules
order generic cleocin 300mg – purchase cefixime sale purchase chloromycetin generic
purchase zithromax generic – cost tetracycline purchase ciprofloxacin pills
amoxicillin buy online – ceftin uk where can i buy baycip
order hydroxyzine 25mg pills – fluoxetine 20mg usa brand endep 10mg
clomipramine 50mg tablet – cymbalta sale doxepin 25mg price
clozaril 100mg drug – cheap tritace pepcid without prescription
order retrovir 300 mg without prescription – buy zyloprim for sale
order glycomet 1000mg – order glucophage 500mg purchase lincocin online
order lasix sale – buy atacand 8mg generic order capoten pill
generic acillin buy monodox for sale cheap amoxicillin without prescription
metronidazole 200mg without prescription – buy zithromax generic buy azithromycin 500mg pills
valacyclovir 500mg uk – diltiazem over the counter acyclovir 800mg pills
buy ivermectin 3mg – buy sumycin generic tetracycline 250mg pills
order ciprofloxacin 500 mg generic – order ciplox 500mg pill purchase erythromycin pills
metronidazole 400mg brand – order generic cleocin 300mg buy zithromax 250mg generic
baycip price – purchase amoxiclav online cheap order augmentin 375mg online
buy generic ciprofloxacin – purchase septra pill order amoxiclav sale
buy atorvastatin sale lipitor 10mg usa oral atorvastatin 10mg