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Cryo-EM 初学者入门资料集锦

发布: 2018-02-14 1,060阅读 169评论

本文由清华大学 宋文飞整理

书籍：

冷冻电镜相关书籍：

《Three-Dimensional Electron Microscopy of Macromolecular Assemblies》 by Joachim Frank

冷冻电镜基本原理书籍：

《Diffraction Physics》Third Revised Edition by John M.Cowley

《电子衍射物理教程》 by 王蓉

《电子晶体学与图像处理》by 李方华

《薄晶体电子显微学》by P. Hirsch

视频：

冷冻电子显微镜（Cryo-EM）入门 by 加州理工学院 Grant J.Jensen

链接：https://www.coursera.org/learn/cryo-em

Eva Nogales 的视频：

链接：https://www.youtube.com/watch?v=nkGRhYv01ag

MRC Lectures:

链接：ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/EM-course

NRAMM Workshops:

链接：http://nramm.nysbc.org/workshops/

NRAMM videos:

链接：https://www.youtube.com/channel/UCgd1dW4HQ_yvpRjFc670htw

CCPEM symposium:

链接：https://sas.stfc.ac.uk/vportal/

Advances in Cryo-EM related books：

Cryo-EM Part A-C & Recent advances in cryo-EM (Methods in Enzymology)

链接： http://www.sciencedirect.com/science/bookseries/00766879/481

http://www.sciencedirect.com/science/bookseries/00766879/482

http://www.sciencedirect.com/science/bookseries/00766879/483

http://www.sciencedirect.com/science/bookseries/00766879/579

Reviews of Cryo-EM：

1.”A primer to single-particle cryo-electron microscopy.” https://www.ncbi.nlm.nih.gov/pubmed/25910204

2. “Structural analysis of macromolecular assemblies by electron microscopy.” https://www.ncbi.nlm.nih.gov/pubmed/21919528

3. Structural Analysis of Protein Complexes by Cryo Electron Microscopy https://link.springer.com/protocol/10.1007%2F978-1-4939-7033-9_28

4. 电子显微三维重构技术发展与前沿 http://www.cnki.com.cn/Article/CJFDTotal-SWWL201007005.htm

论坛：

http://www.bio3dem.com/

数据库：

http://www.rcsb.org/pdb/home/home.do

http://www.ebi.ac.uk/pdbe/emdb/index.html/

http://www.emdatabank.org

软件：

常用计算相关软件：

Relion: http://www2.mrc-lmb.cam.ac.uk/relion

Eman: http://blake.bcm.edu/emanwiki/EMAN2

Gctf / Gautomatch: http://www.mrc-lmb.cam.ac.uk/kzhang

Frealign: http://emlab.rose2.brandeis.edu/frealign

数据收集、后处理等更多相关软件汇总网址：

http://www.emdatabank.org/emsoftware.html

https://en.wikibooks.org/wiki/Software_Tools_For_Molecular_Microscopy

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