您的位置 首页 工具使用

实用SCI绘图教程|用Photoshop处理Western Blot条带

发布: 2017-04-28 1,177阅读 237评论

SCI绘图中最基本的绘图可能就是Western blot条带绘图了，但凡涉及到分子机制的文章中少不了Western blot，本文就介绍下如何从原始的扫描图片绘制得到可发表格式的图片

我们首先来看下本节的任务

 

首先我们来分析下上图从左边的原始扫描图到右边的最终图片需要解决的问题：

（1） 左边图片条带不是水平的，我们需要将其变为水平。

（2） 左边图片条带大小不一，我们需要将其裁剪成一样宽度的条带。

（3） 我们需要对条带进行描边，或者进行明度对比度等调整。

那么接下来我们开始吧。

1. 首先我们打开原始扫描图片，选择矩形框选工具，将三个条带分别复制到新的图层中，框选区域要稍微大一些。

 

 

2. 接下来我们要将条带变为水平，我们选中一个图层，然后按Ctrl+T， 就可以鼠标拖动旋转了。

 

3. 除了上面的让条带变为水平的方式之外，我们还有另外一种方法。我们选中一个图层，然后选择标尺工具。

 

按住左键不放，从条带左边一直拖动到右边，然后点击上面的“拉直图层”

 

这个时候条带也变为水平了：

 

4. 接下来我们要调整条带，让条带的宽度和高度都一致。我们首先选择矩形选框工具，然后选择固定大小，宽度和高度设置一个合适的值，然后在图片中点击鼠标左键，如果大小不合适，需要重新设置宽度和高度。通过键盘方向键可以移动到合适位置。最后按Ctrl+c ,Ctrl+v复制条带。（注意当前框选的区域一定是在当前的图层下，下图中是选中了proteinA图层，如果你选中了proteinB图层，也就是中间那图层，你按Ctrl+C 会提示你当前没有选中区域）。

 

5. 通过上述方法将其三个条带都复制出来，这样条带的大小都是一致的了。

 

6.接下来添加文字。选中文字工具，并设置字体，一般设置Aria，然后点击工作区域，就会新建一个图层，直接输入文字即可。注意不同文字要放置在不同的图层中，所以每次输入一行结束之后，要进行下面的第3步，然后再执行第1步。

 

 

 

7. 上面的图片已经可以作为最终图片。接下来是一些调整，如果您想进行对比度调整，可以如下操作：

7.1 按住SHIFT键，选中这三个条带所在的图层，然后右键，选择合并图层：

 

7.2 然后选择 图像->调整->色阶，

 

7.3 跳动右边三个小三角形的位置，得到如下的图片，可以看出现在的图片比原来的图片更加明亮清晰了

 

8. 如果您想对每个条带进行描边，可以进行如下操作：

8.1 回到第6步结束，不要进行图层合并。

8.2 选中一个图层，然后在图层面板中的这个图中上面右键，选择混合选项。

 

8.3 勾选描边，然后设置大小为2像素

 

8.4 分别对三个图层进行描边处理：

 

1.17K赞
标签:



相关文章

发表回复

您的电子邮箱地址不会被公开。 必填项已用*标注

评论列表(237)

  3. Hi! I know this is kinda off topic but I was wondering if you knew where I could locate a
    captcha plugin for my comment form? I’m using the same blog platform as yours and I’m having problems finding one?
    Thanks a lot!

    Here is my web blog :: blackriverwormfarm.com

    回复

  7. Hello very nice web site!! Man .. Excellent .. Amazing ..
    I’ll bookmark your website and take the feeds additionally?
    I am glad to find a lot of useful information right here in the submit, we want develop extra techniques in this regard,
    thank you for sharing. . . . . .

    Feel free to surf to my web blog … Plan cul à Marseille 05 avec laurence

    回复
  11. 📐 You have received a email № 886. Go > https://telegra.ph/Go-to-your-personal-cabinet-08-25?hs=9b92844f8f2be26e02fc957e38eace5c& 📐说道：

    tdefth

    回复

  14. After exploring a handful of the articles on your web site, I honestly appreciate your way of blogging.
    I added it to my bookmark website list and will
    be checking back in the near future. Please check out my website
    as well and tell me what you think.

    Feel free to surf to my site :: Historique des villes de recherches pour

    回复

  37. “Once you plant that cock of yours in some squirmy hole and empty those warm balls of yours into a moist wet hole. Then you are on your way to becoming a man, and only then will you, come-of-age. But it is only a step onto the winding pathway towards manhood. It is my job to teach you what it means to be a man.” His father stands as he finishes his sentence. Loosening his belt and pulling his shirt out from the tucked confines of his pants. “Coach told us. To go beat off. Seeing all those tents in our shorts; he said we should have all been working out naked. Our shorts weren’t covering up, anything, as our cock’s periscoped out and over the elastic waistbands of our shorts.” He says to his dad as he steps from the shower.

    回复

  40. All the blood rushes from his brain to his throbbing erection plus the heat of the shower, making the young lad, light-headed. That night, I did a whole spa routine to make sure my skin was soft and my ass was stretched and wet. I was wearing nothing but a jockstrap as I was told. Waiting by the door on fourth. My heartbeat was so fast as my mouth was dry. I heard the footstep approaching. I left the door unlocked as one of the instructions I was received from daddy. He stepped in with his size 10 Christian Loublitine loafer where my eyes were going up from there. When I made eye contact with daddy, daddy slapped me across the face and said, “Bad boy, Where the fuck are your sneakers as I told you to wear tonight.” I was trembling and said in a very low voice, “ sorry daddy I fooooorgot.”

    回复

  41. Nicely put, Regards!
    gta online continue to scope out the casino [url=https://usagamblinghub.com/#]best online casinos[/url] how to use paypal for online casino

    回复

  42. You mentioned this wonderfully.
    online casino with gcash [url=https://usacasinomaster.com/#]casino gambling online[/url] fruit party online casino

    回复

  43. He does not hear the slamming of the front door. Neither does he hear the footsteps on the wood floor in the hallway. The shower drowns away all this noise. [url=https://arturzasada.pl/]polskie porno[/url] Daddy was furious as I could hear his deep breath. He sat down on the bench by the door. I was looking down afraid he was going to kick my ass. He lifted my head from my chin using the tip of his loafer where I can smell that smelly sheer socks he has been worn all day. Just when our eyes met, a big stinky manly wad landed on my face. Then I felt a big rough hand rubbing the spit all over my face. I heard the daddy say, “ next time remember what I told you exactly, I don’t want this to happen again, you hear me? I paused and said, “ yeeee…”. Before I finished the sentence, another spit and a big slap on me. “ when I told you something you must say yes. There is no room for you to argue or think, understood?” He said in a deep and firm tone. “ yes sir”. Without missing a beep I said it. “good boy, your night is just about to start.”

    回复

  44. Incredible quite a lot of amazing info!
    ainsworth gaming technology online casinos [url=https://luckyusaplay.com/#]usa casino online[/url] autoexcludere la casino online

    回复

  45. Cheers, I enjoy it.
    tennessee casino online [url=https://luckyusaplay.com/#]online casino sites[/url] online casino paypal zahlung

    回复

  49. With thanks. Numerous information.
    online casino malta [url=https://uscasinoguides.com/#]online casinos usa[/url] batavia casino online

    回复

  51. Nicely put, Regards!
    online casino mobil [url=https://usaplayerscasino.com/#]games casino free online[/url] newest online casinos 2023

    回复

  53. Really many of beneficial data.
    sc casino online [url=https://usagamblinghub.com/#]free online casinos[/url] auszahlung im online casino

    回复

  54. This is nicely said! .
    bbb sugharhouse online casino [url=https://usacasinomaster.com/#]free online games casino[/url] golden nugget casino online gaming

    回复

  55. You revealed that perfectly!
    hopa casino online casino [url=https://luckyusaplay.com/#]new online casinos usa[/url] az online casino sites

    回复

  58. Amazing many of valuable facts.
    online casino apps with free bonus [url=https://uscasinoguides.com/#]online live casinos[/url] online casino betsson

    回复

  59. Thank you. I value this!
    online casino 01.07.2021 [url=https://usaplayerscasino.com/#]top rated online casinos[/url] new casino online no deposit

    回复

  61. Thanks, Loads of forum posts!
    golden nugget mi online casino [url=https://usagamblinghub.com/#]best online casinos that payout usa[/url] vip host online casino

    回复

  63. Nicely put. Regards.
    juegos de casino online mexico [url=https://usacasinomaster.com/#]online casinos no deposit bonus[/url] nj online casinos new

    回复

  64. Appreciate it, A good amount of knowledge!
    australian online casino real money reviews [url=https://luckyusaplay.com/#]free online casinos[/url] no deposit bonus casinos online

    回复

  66. You expressed that terrifically!
    gta online casino vincent [url=https://uscasinoguides.com/#]online casinos with no deposit bonus[/url] gta online casino discount

    回复

  67. You definitely made the point!
    2020 usa online casinos [url=https://usaplayerscasino.com/#]us casinos online[/url] pa online casino launch date

    回复

  69. Position certainly considered..
    nj hard rock casino online [url=https://usagamblinghub.com/#]online live casino[/url] almighty vikings online casino

    回复

  71. Incredible quite a lot of superb advice.
    atlantis casino online slots [url=https://usacasinomaster.com/#]best online usa casino[/url] legit online casino real money no deposit

    回复

  73. Cheers, An abundance of facts!
    ultra panda online casino no deposit bonus [url=https://luckyusaplay.com/#]best online casino[/url] watch casino royale online

    回复

  75. Helpful advice. Regards.
    online casinos for united states [url=https://usaplayerscasino.com/#]casinos online usa[/url] comprar software de casino online argentina

    回复

  77. Kudos! Excellent stuff!
    bayview online casino dealer [url=https://usagamblinghub.com/#]casino gambling online[/url] online casinos indiana

    回复

  81. With thanks! I like it.
    jackpot party casino online free [url=https://luckyusaplay.com/#]free casino online games[/url] online casino gratis guthaben

    回复

  85. You said it perfectly.!
    online casinos com netflix [url=https://uscasinoguides.com/#]new online casinos usa real money[/url] chumba casino online mobile login

    回复

  87. Thank you. Numerous posts!
    1000 bonus online casino luxury casino [url=https://usaplayerscasino.com/#]online casino bonuses[/url] casino europa online

    回复

  89. You actually said it effectively.
    best online sportsbook casino [url=https://usagamblinghub.com/#]bet online casino[/url] create an online casino

    回复

  90. Wow many of beneficial information.
    online casinos italia [url=https://usacasinomaster.com/#]online casino top[/url] best uk online casino no deposit

    回复

  94. Nicely put, Cheers!
    mohegan sun nj online casino bonus code [url=https://usaplayerscasino.com/#]best online casino no deposit bonus[/url] maltese online casino site

    回复

  96. Whoa tons of useful information.
    new online casinos that pay real money [url=https://usagamblinghub.com/#]new casino online[/url] online casino roulette strategy

    回复

  98. Whoa tons of superb advice!
    how to tell if online casino is legit [url=https://usacasinomaster.com/#]casino live online[/url] lucky 777 online casino philippines

    回复

  100. Cheers. Loads of information.
    miglior casino online non aams [url=https://luckyusaplay.com/#]casino online no deposit[/url] galaxy game online casino

    回复

  102. You actually expressed it fantastically.
    bestes online casino echtgeld [url=https://usaplayerscasino.com/#]best online casino[/url] planet 7 online casino no deposit bonus codes

    回复

  103. This is nicely said. !
    online casino 400 willkommensbonus [url=https://usagamblinghub.com/#]new online casino no deposit bonus[/url] capital one online casino

    回复

  106. You actually said this fantastically.
    advanzia kreditkarte online casino [url=https://luckyusaplay.com/#]casinos gambling online[/url] 10 aud deposit online casino

    回复

  107. Incredible a good deal of amazing data!
    pulse casino online [url=https://uscasinoguides.com/#]united states online casino[/url] online canlД± casino

    回复

  109. Amazing a lot of useful advice.
    milkyway casino online [url=https://usaplayerscasino.com/#]new online casinos for usa[/url] pay real cash online casinos

    回复

  111. Really many of superb advice.
    jackpot city online casino free [url=https://usagamblinghub.com/#]best online casinos[/url] stations online casino

    回复

  152. Very nice post. I just stumbled upon your weblog and wished to say that I’ve truly enjoyed surfing around your blog posts. After all I will be subscribing to your rss feed and I hope you write again very soon!

    https://youtu.be/TXUHPP1XCaY

    回复

  154. Wow! This could be one particular of the most helpful blogs We’ve ever arrive across on this subject. Basically Wonderful. I am also an expert in this topic therefore I can understand your effort.

    https://youtu.be/4qmYg4go6Mo

    回复

  162. I do like the manner in which you have presented this particular concern plus it does supply me personally some fodder for thought. On the other hand, through what I have personally seen, I only hope as other commentary pack on that individuals continue to be on issue and not embark upon a tirade associated with some other news of the day. Yet, thank you for this outstanding point and though I do not necessarily agree with this in totality, I respect your standpoint.

    https://gullybetappdownload.com/

    回复

  166. I know this if off topic but I’m looking into starting my own weblog and was wondering what all is required to get set up? I’m assuming having a blog like yours would cost a pretty penny? I’m not very internet smart so I’m not 100 positive. Any suggestions or advice would be greatly appreciated. Appreciate it

    https://youtu.be/sc7Ltb-k2As

    回复

  172. I do enjoy the manner in which you have presented this specific difficulty plus it does indeed supply me personally a lot of fodder for consideration. Nevertheless, because of what precisely I have experienced, I really trust when other feedback stack on that folks remain on point and in no way embark on a soap box associated with some other news du jour. All the same, thank you for this fantastic point and while I can not necessarily agree with this in totality, I respect your point of view.

    https://youtu.be/vFFWhl3V5so

    回复

  173. Nice read, I just passed this onto a colleague who was doing a little research on that. And he just bought me lunch since I found it for him smile So let me rephrase that: Thank you for lunch!

    https://newadm4d.com/

    回复

  174. Thanks a lot for providing individuals with a very splendid chance to read from this site. It really is so useful and also full of fun for me and my office colleagues to visit your web site really three times in one week to see the fresh things you will have. And indeed, I’m always impressed concerning the remarkable information served by you. Selected 2 facts on this page are truly the most suitable we have all ever had.

    https://spotigeek.com/

    回复

  176. Dead written subject matter, thank you for information. “He who establishes his argument by noise and command shows that his reason is weak.” by Michel de Montaigne.

    https://youtu.be/vBx53052Too

    回复

  186. I have been absent for a while, but now I remember why I used to love this site. Thank you, I?¦ll try and check back more frequently. How frequently you update your site?

    http://www.tlovertonet.com/

    回复
联系我们

联系我们

(44)07934433023

在线咨询: QQ交谈

邮箱: info@bioengx.org

关注微信
微信扫一扫关注我们

微信扫一扫关注我们

关注微博
返回顶部