大家好，我是风烬辞，之前在《实验万事屋》这个公众号上发过若干文章的（目前合作已终止）、奖学金只有一块钱（还没给我）在若干实验室漂流的大四妹子……
很快就要稀里糊涂毕业了，还没来得及伤感一下，发现论文还没写，伤感的乌云又笼罩了一层。有着段子手的秘制（迷之）自信的我决定毕业前一个月再开始赶论文，现在先跟你们显摆一下我在这半年的毕设期间干了哪些不务正业的事情……
我的毕设是干啥呢？我是要用大肠杆菌（E.coli）诱导表达三个蛋白，质粒可爱的师姐已经帮我构建好了，我只要进行转化诱导表达就行了。
在三周的转化失败后，我终于得到了可以表达我目的蛋白的小菌菌，于是！为了迎接来之不易的蛋白，我专门设计了一个纯化箱！如下图：
你们以为这就是全部吗？——当然不，这可是我的心血大作！还有半自动进样装置什么的就是不给你们看！要看完整的蛋白纯化箱请给我发十次奖学金（每次一块钱……分期付也是可以的啊同学~π_π）！
好吧再给你们露一个羞羞的构造……
这是用来连接两条EP管传送带的构造，看懂的男同学都是有故事的男同学@_@。
下面是技能干货……
超声破碎细胞的时候，圆底管像这样摇摇晃晃绕着超声杆跳钢管舞？
就这样捆起来就好了嘛……感谢泡沫板橡皮筋以及长尾夹同学!
然后纯化的时候，可以像这样在柱子填充物的上方加一个垫片，这个垫片可以用一根铁丝从其他的柱子里面顶出来，能够起到防止液体冲床的作用，这个图片中有气泡，理论上最好不要有气泡，不过这样你们可以很清楚得看到气泡上面就是那个垫片。
当一切就绪之后……我发现老师的蛋白纯化装置风格是这样的:
这翩翩起舞的蝴蝶是闹哪样？！老板你心里一定是住着一个小公举！不过这简陋程度真的超乎了我的想象……
嗯然后我就这样把目的蛋白纯化出来了。
然而，等我把三个蛋白都集齐，到了可以召唤毕业论文的时刻，师姐突然告诉我：“三个蛋白中的一个……当初设计引物的时候就设计错了，错了一个氨基酸，所以……那个蛋白……没有用……”
天、呐、没！有！！用！！！
看来我要在这里多待一段时间了……
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